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Abstract

Anthers of nicotiana tabacum cv. Virginica were cultured and the development of pollen grains studied. A large proportion of pollen grains degenerated in culture , the surviving ones belonging to mitotic and binucleate class producd plantlets which originated from’S’ grains. Cold pretreatment of anthers increased both the number of anthers producing plantlets and the number of plantlets per anther. Dividing the culture period into an initiating and a developmental stage proved beneficial. Addition of mannitol and glutamate gave maximum number of plantlets, absence of which reduced yield. Role of mannitol and glutamine in the development of plantlets from pollen grains in anther culture is discussed.

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