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Abstract

Papaver somniferum L. seedlings produced a light green, soft and friable callus when cultured on 1/2 Murashige and Skoog (MS) medium containing 2, 4-D( 0.5 mg/1) and Kinetin (0.1mg/l) . After 2-3 subcultures, callus pieces transferred to liquid medium and placed on orbital shaker showed active growth and formation of many cell clumps with loosely arranged cells. Structures resembling various stages of embryo development found in suspension cultures when transferred on solid MS medium containing Benzylaminopurine (0.5 mg/l), Adenosine sulphate (25 mg/l) and Gibberellic acid (10 mg/l) gave rise to green globular embryoids.

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