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PJB-2001-31
PEG-MEDIATED SOMATIC HYBRIDIZATION STUDIESIN SUGARCANE (SACCHARUM SPP. HYBRID CVS. CoL-54 AND CP-43/33)
Abstract
Somatic hybridization studies were performed using leaf mesophyll as well as homogeneous cell suspension-derived protoplasts. The optimum results were achieved when leaf mesophyll protoplasts from cv. CP-43/33 and homogeneous cell suspension-derived protoplasts from cv. CoL-54 (at 2.0x10 5 ml-1 final density) were used. Polyethylene glycol (PEG) at 35% level was found optimum for maximum protoplast agglutination (32%). Maximum heterokaryon formation (17%) was obtained employing 50 mM l-1 CaCl2.2H20 at pH 10.5 as an eluting solution. The hybridized protoplasts were cultured to monitor the divisions and further growth.
