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PJB-2000-31
OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF L-DOPA (L-3, 4 DIHYDROXY PHENYL ALANINE) FROM L- TYROSINE BY ASPERGILLUS ORYZAE
Abstract
The present study is concerned with the optimization of different cultural conditions for the production of L-DOPA from L-Tyrosine by surface culture technique. Of the 12 different isolates of Aspergillus oryzae tested, for production of mycelium, strain ISB-9 was found to give maximum yield of L-DOPA (0.34 mg/ml) L-DOPA production was maximum at pH 3.0 of the reaction mixture, 50oC, 50 minutes and 2.5 mg/ml, optimum temperature, time and L- Tyrosine concentration, respectively.
