PJB-2015-163
STUDIES ON THE CHANGES OF PROTEIN SECONDARY STRUCTURE AND CARBOHYDRATE CONTENTS IN SEEDLING-STAGE OF ABRUS CANTONIENSIS HANCE IN DROUGHT STRESS BASED ON FTIR AND CHEMOMETRICS
FANG LAN1, DEXIN KONG3, YANQUN LI4 AND RONGSHAO HUANG2*
Abstract
To study the response mechanism of seedling-stage Abrus cantoniensis to drought stress from the level of infiltration conditions, this research uses Fourier infrared spectroscopy based on such mathematical approaches as Fourier self-deconvolution and Gaussian curve-fitting to analyze the broad characteristic absorption bands of the protein secondary structure and carbohydrates in the root, stem and leaf of seedling-stage under four drought stress treatments. The results show that the relative absorption of every type of secondary structure (namely, α-helix, β-sheet and β-turn), the α-helix/β-sheet ratio and the relative contents of starch, soluble sugars and cellulose show significant differences in the different parts of seedling-stage A. cantoniensis under different drought treatments. These differences provide a theoretical basis for investigating the response mechanisms of seedling-stage A. cantoniensis to drought stress and for advancing rational cultivation measures for this species. A new method that is fast, efficient and sensitive is presented for the study of the response mechanism of seedling-stage A. cantoniensis to drought stress.
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