PJB-2012-303
COMPARATIVE SEED STORAGE PROTEIN PROFILING OF MUNG BEAN GENOTYPES
AMJAD HAMEED1*, MEMUNA QURESHI2, MUHAMMAD NAWAZ2AND NAYYER IQBAL1
Abstract
Seed storage protein profiling of mung bean genotypes was performed by SDS-PAGE. Total soluble seed proteins from mung bean seeds were resolved on 12% gels in 22 protein polypeptide bands with molecular weights ranging from 16 to 103 kDa. Very low level of variability was observed in tested genotypes. Dendrogram based on electrophoretic data grouped the 13 genotypes into three clusters at 93% homology. A total of 5 genotypes (NM 13-1, NM 2006, NM 92, Chakwal 97 and Mung 88) were grouped together in the cluster-1. The 2nd cluster consisted of one genotype i.e. AZRI 2006 which was most divergent in the tested germplasm. Seed storage peptide i.e., MSSP-19 differentiated the genotype, AZRI 2006 from rest of tested genotypes and thus can be used for its identification. Remaining seven genotypes grouped together in the 3rd cluster. Based on seed storage protein marker i.e. MSSP-82, NM 2006, can also be differentiated from one of its parent genotype i.e., NM 92. Uniprot and NCBI protein databases were searched for already reported and characterized seed storage proteins in mung bean. Based on information retrieved from the databases and search engines, the resolved protein peptides mainly belong to 8S and 7S vicilin
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