PJB-2012-254
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERIZATION OF LESPEDEZA BICOLOR TURCZ, FROM KATLANG REGION DISTRICT MARDAN, PAKISTAN
SAMIULLAH, ASGHARI BANO* AND RIZWANA ALEEM QURESHI
Abstract
Lespedeza bicolor Turcz commonly called bicolor, bush clover or bicolor lespedeza of the family Papilionaceae has been collected from high saline field of Ditrict Mardan, Pakistan (34° 05’ to 34° 32’ north latitudes and 71" 48’ to 72° 25’ east longitudes). Field capacity of bulk soil was reported 14-16.5%, with EC: 4.1dS/m and pH: 8.5-9.2. Present research reveals bioremediation method to be the most environmentally sustainable method in dealing with soil salinity. According to the plant assessment for mineral uptake and heavy metal accumulation in root, stem and leaves of Lespedeza bicolor, Na+, Ca+2 and Mg+2 were much accumulated whereas, K+ ion accumulation was reported least whereas, among the micro minerals Fe+2, Cu+3 and Mn+2 were found higher than Zn+2, Cr+3, Co+3, Pb+4, Ni+3 and Cd+2. Chlorophyll a content of Lespedeza bicolor leaves was 2-fold higher than chlorophyll b content whereas, high sugar and carotenoid content was reported in Lespedeza bicolor leaves at vegetative stages. Lespedeza bicolor appears to use proline and sugar as osmolyte for osmotic adjustment. Among the enzymes study Peroxidase (POD) and Catalase (CAT) activities were found higher in leaves of Lespedeza bicolor as compared to superoxide dismutase (SOD) and ascorbate peroxidase (APOX). The production of abscisic acid (ABA) was significantly higher as compared to indole acetic acid (IAA) in the selected plant.
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