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MICROPROPAGATION AND GREENHOUSE CULTIVATION OF SCROPHULARIA TAKESIMENSIS NAKAI, A RARE ENDEMIC MEDICINAL PLANT
Abstract
A protocol was developed for direct shoot regeneration of Scrophularia takesimensis using shoot tip explants. The explants were excised from mature field grown plants and cultured on Murashige and Skoog (MS) medium with different concentrations of 2-isopentyl adenine (2-iP), 6-benzyl adenine (BA) or thidiazuron (TDZ). Among the three cytokinins studied, BA was found to be the most effective cytokinin for multiple shoot induction. Addition of auxin to the shoot regeneration medium significantly enhanced the percentage of shoot induction and number of shoots per explant. The greatest percentage of shoot induction was achieved when shoot tip explants were cultured on MS medium supplemented with 3.0 mg L-1 BA and 1.0 mg L-1 indole-3-acetic acid (IAA) with an average of 13 shoots per explant. The regenerated shoots were transferred to modified MS medium supplemented with 3% (w/v) sucrose
