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BIOSYNTHESIS OF EXTRACELLULAR LIPASES BY BACILLUS SP. (MBLB-3) IN RELATION TO THE NUTRITIONAL CONDITIONS
Abstract
The present study deals with the microbial biosynthesis of lipases by a strain of Bacillus sp. Fifteen strains were isolated from oily products. These strains were screened for the production of lipases by solid state fermentation in 250mL Erlenmeyer flasks. Of all the strains examined, MBLB-3 gave maximum production (20.4 ± 0.2a U/g) of lipases. Different agricultural by-products such as wheat bran, rice husk, almond meal, cotton seed meal, soybean meal, sunflower meal and mustard meal were used as substrates. Maximum extracellular lipase activity (33.9 ± 0.5a U/g) was observed when almond meal was used as the substrate and it was moistened with phosphate buffer (pH.8.0). The reaction was carried out by taking bacterial cells as a source of lipases in solid substrate fermentation. 0.5 % of Tween 80 was optimized for the maximum production (42.58 ± 0.8a U/g) of lipases. The above results revealed that the Bacillus lipase can be a good additive to be used in detergents.
