PJB-2011-120
INTER AND INTRA-SPECIFIC VARIATION IN SDS-PAGE OF SEED PROTEINS OF THREE POA L. (POACEAE) SPECIES
AHMET TAMKOC1 AND EMINE ARSLAN2*
Abstract
Total seed storage proteins were analyzed in genotypes belonging to three Poa species collected from Turkey. In this study, SDS-PAGE method was preferred to inter and intra-specifically determine the genetic relationships of Poa species in order to facilitate genotype selection in breeding programs. Individual electrophoregrams of the species obtained with SDS-PAGE can be used as password data for their genetic relationships. Polypeptide patterns ranged from 10 kDa to 128 kDa were calculated according to Nei homology using Bio1D++ computer program. Genetic and morphological differences between the Poa species are discussed. The dendrogram obtained with UPGMA clustering method indicated a low intra-specific genetic diversity while P. pratensis and P. trivialis genotypes stated closer relation as compared to P. angustifolia genotypes. It was concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and genetic relationships of Poa species. Additionally, this method can be useful to determine the correct starting material for plant breeding.
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