Paper Details

PJB-2011-115

ALGINATE PRODUCTION BY A MUTANT STRAIN OF AZOTOBACTER VINELANDII USING SHAKE FLASK FERMENTATION

ZAHID ALI BUTT, IKRAM-UL-HAQ AND MUHAMMAD ABDUL QADEER
Abstract


The present study describes the improvement of parent strain of Azotobacter vinelandii NRRL-14641 for enhanced alginate production using shake flask fermentation. Results indicated that a mutant A. vinelandii EMS-45 obtained after EMS treatment gave 1.55 fold better alginate productions than that of the parent strain. Different cultural conditions were employed to optimize the polymer production in the fermentation broth. It was noted that maximum alginate production (6.17 g/l) was obtained with nitrogen rich phosphate limited medium (NRP) after 110 h of incubation period. The incubation temperature 30°C, pH 7.0, agitation intensity 200 rpm, carbon and nitrogen source were also optimized and sucrose and peptone were found as the best to support 1.55 fold higher alginate than that in un-optimized medium.

To Cite this article:
Download

We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More

Get In Touch

© 2022-23 Pakistan Journal of Botany. All Rights Reserved