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GENOTYPE INDEPENDENT IN VITRO REGENERATION SYSTEM IN ELITE VARIETIES OF SUGARCANE
Abstract
Nine sugarcane genotypes were tested for callus induction and subsequent regeneration on MS medium supplemented with different combinations of hormones. Genotype-independent plant regeneration was developed from calli induced from young leaf roll discs on MS medium supplemented with seven different levels (1-5mgl-l) of 2, 4-D. Further, five levels (1-5mgl-l) of 2, 4-D alongwith 1 mgl-1 of Kinetin for callus induction were used. All the genotypes responded well to 2, 4-D alone as well as in combination with Kinetin. Nevertheless, high percentage of regeneration was observed on MS medium supplemented with 500mg l-l casein hydrolysate. Regenerated shoots developed profused roots on NAA (4-5mgl-l) and casein hydrolysate (500mgl-l)-containing MS medium which were otherwise delayed on a medium containing full as well as half strength MS salts.
