PJB-2010-190
HIGH FREQUENCY REGENERATION SYSTEM OPTIMIZATION FOR WHEAT CULTIVAR INQILAB-91
ASMA AFZAL1, HAMID RASHID2*, M. HAROON KHAN2, ZUBEDA CHAUDHRY3 AND SALMAN A. MALIK1
Abstract
In the present study, a high efficiency regeneration system was developed in wheat (Triticum aestivum L.) cv. Inqilab-91. Mature embryos were taken as explant source and inoculated on MS media for callus induction. MS media with different concentrations of 2, 4-D (2mg/l, 3mg/l and 4mg/l) were used for callus induction. Maximum percentage (76.04%) of callus induction was achieved with 3mg/l of 2, 4-D whereas 53.12% and 56.25% of calli were formed with 2mg/l and 4mg/l of 2, 4-D respectively. Four different hormones (IAA, BAP, Kn and 2ip) were used in different concentrations and combinations in MS medium for regeneration from calli of mature embryos. The regeneration frequency (46.66%) was obtained with 0.1mg/l of IAA and 0.5mg/l of BAP. Different concentrations of Kn (0mg/l, 0.5mg/l
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