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PJB-2010-167

IN VITRO PRESERVATION OF PYRUS GERMPLASM WITH MINIMAL GROWTH USING DIFFERENT TEMPERATURE REGIMES

MAQSOOD AHMED1*, MUHAMMAD AKBAR ANJUM2, ASAD HUSSAIN SHAH3 AND ABDUL HAMID1

Abstract

In vitro cultures of 9 pear genotypes collected from different areas of Azad Jammu & Kashmir were established on MS medium supplemented with 30 g l-1 sucrose, 7 g l-1 agar and 1 mg l- BAP. Proliferated shoots of these genotypes were excised aseptically, cultured on fresh medium and tested for their survival and regenerative ability at various incubating temperatures regimes i.e.

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