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DEVELOPMENT OF AN EFFICIENT REGENERATION PROTOCOL FOR THREE GENOTYPES OF BRASSICA JUNCEA
Abstract
Two phytohormones, auxins (Naphthalene acetic acid and Indole acetic acid) and cytokinins (Benzylaminopurine and Kinetin) with concentrations were used to develop an efficient regeneration protocol for 3 genotypes of Brassica juncea (UCD-635, RL-18 and NIFA RAYE). The explants were cultured on MS-medium supplemented with BAP 1.0 mgL-1/NAA 0.1 mgL-1, BAP 2.0 mg L-1/NAA 0.2 mg L-1, BAP 3.0 mgL-1/NAA 0.3 mg L-1 and Kinetin 1.0 mg L-1/IAA 0.1 mg L-1, Kinetin 2.0 mg L-1/IAA 0.2 mg L-1, Kinetin 3.0mg L-1/IAA 0.3 mg L-1. Maximum callus production (65.55) was observed on MS medium containing with BAP 2.0 mgL-1/NAA 0.2 mg L-1. Maximum shooting ( 22.31) was observed BAP 3.0 mg L-1/NAA 0.3 mg L-1 and KIN 3.0 mg L-1/IAA 0.3 mg L-1. Regeneration efficiency was found maximum (7.13) with BAP 3.0 mg L-1/NAA 0.3 mg L-1. The three genotypes were found significantly different at p≤0.05 in shoots production and regeneration efficiency.
