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Paper Details

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PJB-1995-51

PLANTLET REGENERATION FROM MATURE EMBRYOS OF PEANUT (ARACHIS HYPOGAEA L.)

IHSAN ILAHI, ROBINA SAMI AND MUSSARAT JABEEN

Abstract

Mature embryos, excised from the soaked peanut seeds, when cultured on MS medium supplemented with BA and 2,4-0 each @ 0.5 mg/I induced, callus. Regeneration occurred when this callus was subcultured on to MS containing BA and kin each @ 1.0 mg/I. Similarly, development of callus followed by regeneration was noticed in mature embryos in MS medium containing 0.5 mg/l kin and 1.5 mg/l NAA. Direct regeneration was also observed on MS containing 1.0 mgll each of BA and kin. All the regenerated shoots when transferred to the rooting medium (1/2 MS) supplemented with 0.1 mg/I NAA showed development of root.

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