PJB-2008-276
MOLECULAR AND BIOCHEMICAL ASSESSMENT OF BRASSICA NAPUS AND INDIGENOUS CAMPESTRIS SPECIES
S. J. ABBAS1, FARHATULLAH2, I. A. KHAN1, KHAN BAHADAR MARWAT3 AND IQBAL MUNIR1
Abstract
In Pakistan, Brassica is the second most important source of oil after cotton. It contributes about 17% to the domestic production of edible oil. Parental lines along with five F2s were assessed for biochemical parameters using Near Infrared Reflectance Spectroscopy (NIRS). Parental lines contain more oil 45.85% as compared to F2s 42.26% while the F2s contain more protein 25.92% as compared to the parents 23.70%. Both parents and F2 contain high glucosinolate and fatty acids contents. Insulin Growth like Factor (IGF) primer sets were used to estimate genetic relationship among 5 F2 segregating population of Brassica along with 9 parental lines. On an average 29 alleles were amplified using IGF primer sets. Mean genetic distance estimates ranged from 0.25-1.00 (G.D = 0%-100 %), respectively. Size of scorable fragments ranged from approximately 250 to >2000 bp. A high level of genetic dissimilarity (GD= up to 100%) was estimated among all genotypes. Entries were grouped in clusters using cluster analysis. On the basis of dendrogram, most diverse genotypes were identified which should be utilized in further breeding programs aimed at creating genetic variability in local germplasm.
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