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PJB-2007-309
OPTIMIZATION OF VOLUME OF FERMENTATION MEDIUM FOR THE PRODUCTION OF ALKALINE PROTEASE BY AN EMS MUTANT STRAIN OF BACILLUS SUBTILIS IH-72
Abstract
The study deals with the effect of volume of culture medium on the enhanced production of alkaline protease by an EMS-mutant culture of Bacillus subtilis in a lab scale bioreactor and shake flasks. The laboratory scale bioreactor of 7.5 L capacity was employed for the microbial cultivation experiments in addition to 250ml Erlenmeyer flasks. The parental strain of Bacillus subtilis was improved by inducing mutations using Ethyl methane sulphonate (EMS). After EMS-mutagenesis
