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IN VITRO RE-GENERATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SHOOT TIPS OF MATURE TREES
Abstract
Micropropagation of guava through shoot tip culture from five years old bearing plants was carried out at Nuclear Institute for Food & Agriculture, Peshawar. Shoot tips after sterilization was cultured on modified MS medium supplemented with different concentrations and combinations of BAP and L-glutamin. Maximum number of shoots (72 %) was developed in to plantlets when MS was supplemented with BAP 1 mg/L and 500 mg/L L-glutamine. MS control and BAP 1mg/L combined with glutamine 250 mg/L gave minimum (22%) plantlets. Maximum (3.5) number of shoots per culture was found in 0.5 mg/L BAP alongwith 500 mg/L L-glutamine. MS supplemented with 2.5 mg/L IAA + 2.5 mg/L IBA gave maximum (54) plants rooted with average number of roots (3.8) per plantlet. The In-vitro produced plantlets were successfully established in soil.
