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Application of Zinc Oxide Nanoparticles Immobilizes the Chromium Uptake in Spinach (Spinacia oleracea L.) by Regulating the Physiological and Biochemical Attributes
Abstract
Nanoparticles are emerging technique and are used to control the biotic and abiotic stress. They are highly specific to their targeted site. Zinc oxide nanoparticles (ZnO NPs) have been used as a new way to control plant tolerance and reduce heavy metal stress, but further research is needed since we don't fully understand how ZnO NPs can reduce chromium (Cr) stress, particularly in spinach. In our current experiment, we used two levels of green synthesis-based ZnO NPs (300ppm and 600ppm) to alleviate the chromium stress. Spinacia oleracea L.is worldwide cultivated vegetable with a leafy structure and it is affected by heavy metals. Chromium affects the spinach here we studied the two varieties of spinach with different level of chromium stress (50mM and 100mM) concentration. The study showed that chromium stress affects the spinach plant by affecting biomass of plant their fresh and dry weight reduced and chlorophyll contents also decreased. SOD, POD, CAT, H2O2 and MDA increased in stressed plants. While applying nanoparticles of 600pm showed to normal growth by alleviating the heavy metal stress. Plant biomass of stressed plant increased and chlorophyll contents also increased by reducing the enzymatic activity SOD, POD, CAT and hydrogen peroxide and MDA.
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