Paper Details

Abstract

Abstract This study aimed to Use micro-morphological and molecular techniques, characterize six landrace varieties of common bean "Phaseolus vulgaris L." seeds.The goal was to understand their geographic distribution and tolerance to improve the benefits from this important economic crop. The chosen bean varieties are Phaseolus vulgaris L. var Bronco, Phaseolus vulgaris L. var Giza 15, Phaseolus vulgaris L. var Giza Red, Phaseolus vulgaris L. var paulista, Phaseolus vulgaris L. var samantha, and Phaseolus vulgaris L. var sapares. We determined the seed widths and lengths with a Leica EZ4 microscope and a digital caliper. The morphological characteristics of the seeds were observed under a stereomicroscope. This occurred for both discrete traits, such as seed color and shape, and quantitative traits, for example, seed weight, length and width. The surface morphology of the bean seeds was studied using Scanning Electron Microscopy (SEM). SCoT markers revealed genetic variability in the analyzed varieties. A total of 124 DNA bands were detected by molecular markers from 12 SCoT primers, of which 44 were polymorphic. The average rate of polymorphism was 35.48%. Three clusters were formed according to genetic distance revealing a high degree of genetic divergence in the beans

To Cite This Article