Paper Details

Abstract

Valeriana officinalis L. is an important medicinal plant widely used for sedative and neuroactive applications. However, limited studies have integrated optimized adventitious root culture with downstream extraction process optimization for efficient utilization of V. officinalis biomass. To establish an efficient In vitro production and utilization route for V. officinalis, Single-factor experiments and Box–Behnken response surface methodology were applied to optimize adventitious root induction, suspension culture, and Soxhlet extraction conditions. Our results indicated that adventitious roots in V. officinalis were efficiently induced using 0.2 mg·L⁻¹ NAA combined with 0.05 mg·L⁻¹ 6-BA. The culture conditions of 42 g·L⁻¹ sucrose, 39.854 mmol·L⁻¹ total N, and 1.590 mmol·L⁻¹ total P were suitable for adventitious root growth. Using adventitious roots produced under these conditions as the raw material, optimization of the Soxhlet extraction process gave a petroleum ether extract yield of (3.13 ± 0.12) %. The developed response surface models were statistically significant (p<0.0001) with non-significant lack-of-fit. In summary, this study established an integrated experimental framework from adventitious root induction and suspension culture to the preparation of lipophilic extracts. Although chemical profiling of valerenic acid-related compounds was not performed in this study, the optimized system provides a basis for future phytochemical investigations. Bioactivity evaluation of the obtained extracts remains necessary for confirming functional relevance. The optimized culture and extraction conditions provide a reproducible platform for future phytochemical characterization and large-scale utilization of V. officinalis adventitious roots.

To Cite This Article