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Abstract

Valeriana officinalis L. is a medicinal plant with notable sedative, antidepressant, neuroprotective, hepatoprotective, antitumor, antispasmodic, anti-inflammatory, antioxidant, and antibacterial activities. However, the production efficiency and utilization value of its root-derived products are strongly affected by culture conditions and extraction processes. To establish an efficient in vitro production and utilization route for V. officinalis, single-factor tests, two-factor combination tests, and a Box–Behnken design were used to optimize adventitious root induction, adventitious root suspension culture, and the extraction process for petroleum ether extracts. Our results indicated that adventitious roots in V. officinalis were efficiently induced using 0.2 mg·L⁻¹ NAA combined with 0.05 mg·L⁻¹ 6-BA. The culture conditions of 42 g·L⁻¹ sucrose, 39.854 mmol·L⁻¹ total N, and 1.590 mmol·L⁻¹ total P were suitable for adventitious root growth. Using adventitious roots produced under these conditions as the raw material, optimization of the Soxhlet extraction process gave a petroleum ether extract yield of (3.13 ± 0.12)%. In summary, this study optimized a continuous technical route from adventitious root induction and suspension culture to the preparation of lipophilic extracts. These results may provide a reference for in vitro production, further component analysis, and resource utilization of V. officinalis.

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