PJB-2026-106
Genetic affinity of turfgrass-type tall fescue (Festuca arundinacea Schreb.) populations from the Central Black Sea Region revealed by ISSR markers
Dr. Fatih ALAY
Abstract
Tall fescue (Festuca arundinacea Schreb.) is an important cool-season turfgrass species due to its wide adaptability and tolerance to environmental stresses. Understanding the genetic relationships among naturally occurring genotypes is essential for effective breeding and conservation strategies. This study aimed to assess the genetic affinity among turfgrass-type tall fescue genotypes collected from the natural flora of the Central Black Sea Region of Türkiye using ISSR molecular markers. In addition, PCR temperature and amplification time parameters were re-optimized to improve marker reproducibility.
A total of 49 turfgrass-type genotypes were analyzed using the ISSR-PCR technique. Molecular analyses were conducted at the Laboratory of the Faculty of Agriculture, Department of Agricultural Biotechnology, Ondokuz Mayıs University (Samsun, Türkiye). ISSR primers generated clear and reproducible banding patterns with fragment sizes ranging from 200 to 1500 bp. In total, 147 bands were scored, and complete polymorphism (100%) was observed for primers ISSR4, ISSR5, and ISSR14. The polymorphic information content (PIC) values varied between 0.177 and 0.208, indicating moderate to high discriminatory power of the primers used.
Genetic similarity analysis revealed substantial variation among the genotypes. The most genetically distant genotype pairs included FA37-02 with FA09-19, FA09-08, FA22-11, FA24-18, and FA32-17, as well as FA36-13 with FA11-12, FA20-07, and FA24-03. The average genetic similarity coefficient was 0.639, reflecting a considerable level of genetic diversity within the studied material.
The results demonstrate that turfgrass-type tall fescue genotypes from the Central Black Sea Region possess a broad genetic base. These findings provide valuable information for future turfgrass breeding programs and contribute to the conservation and sustainable utilization of tall fescue genetic resources.
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