PJB-2026-44
Effectiveness of Secondary Metabolites in Chrysanthemum coronarium on Bemisia tabaci, Tetranychus turkestani and Aphis craccivora
Enas Hamed
Abstract
Insecticidal activity of secondary metabolites derived from Chrysanthemum coronarium, also called the cow's eye or crown daisy plant, is investigated in this work in relation to the emergence of the whitefly (Bemisia tabaci), a major agricultural pest, as nymphs and pupae, Tetranychus turkestani and Aphis craccivora. Ethanol, diethyl ether, and ethyl acetate solvents were used to prepare plant extracts from the leaves and flowers, and their effectiveness was assessed at different concentrations (20, 200, and 1000 ppm).Gas chromotoghraphy for ethanolic extract of leave and flowers was used to evaluate the active compounds found in each explant, it revealed the existence of bioactive substances like supporting their function in insecticidal activities with alkaloids (Vinblastine, Vincristine), flavonoids (Quercetin, Kaempferol), and phenolic acids (Caffeic and Ferulic acids). According to bioassays, the highest dose (1000 ppm) was the most effective, causing a 71.7% inhibition of pupal emergence and a 75.1% mortality rate for whitefly nymphs. Ethyl acetate extract had the greatest effect in preventing pupal emergence among the solvent extracts, but diethyl ether and crude extracts showed the best larvicidal effects. The results demonstrate C. coronarium's potential as a natural pesticide source, providing a sustainable substitute for manufactured chemical insecticides.
The objective of this study is to determine whether chrysanthemum flower extract and its fractions have biological activity against whitefly (Bemesia tabaci), Bemisia tabaci, Tetranychus turkestani and Aphis craccivora mortality and whether they may be considered botanical insecticides. Additionally, assess their safety levels in relation to Microplitis rufiventris, one of its effective larval parasitoids. Additionally, Gas Chromatography and Mass-Spectrum (GC-MS) is used to identify the active chemicals in the active fractions.