PJB-2026-33
Integrated Molecular Marker and Biochemical Profiling of Vegetable Genotypes Reveals Genetic Diversity and Stress Adaptation under Semi-Arid Conditions
Dr. Mevlüde Tatar
Abstract
Understanding the molecular basis of stress adaptation and genetic diversity in vegetable crops is essential for developing climate-resilient cultivars under increasing environmental constraints. However, integrative studies combining molecular marker–based genetic characterization with biochemical profiling under realistic semi-arid conditions remain limited. Such integrated approaches provide valuable insights into genotype differentiation, adaptive potential, and breeding-oriented selection strategies. In this study, vegetable genotypes were evaluated using an integrated molecular and biochemical framework under semi-arid, water-limited field conditions. Biochemical traits associated with stress response and quality were assessed alongside molecular diversity analyses based on ISSR, SSR, and SNP markers. Multivariate approaches were applied to characterize genotype differentiation and response patterns under environmental stress. The results revealed pronounced genotype-dependent variation in both biochemical profiles and molecular structure. SNP-based principal component analysis clearly separated genotypes according to their genetic background, while ISSR–SSR clustering supported these patterns of differentiation. Biochemical trait responses further indicated distinct metabolic adjustment strategies among genotypes exposed to water-limited conditions. Overall, the integration of molecular marker analyses with field-based biochemical profiling provides a robust and complementary basis for discriminating genotypes under semi-arid environments. The findings highlight the potential of integrative biotechnology-driven approaches to support the identification of stress-resilient genotypes and to enhance breeding strategies aimed at sustainable vegetable production under increasing climate variability.
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