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Abstract

This study used a starter strain, Bacillus velezensis TS01, isolated from distiller’s grains. To directionally breed thermotolerant strains at 42-46°C for 532 h and 50 generations, EVOL (cell adaptive evolution technology) was used. Strain 46-1 exhibits a 250% increase in growth compared to the original strain at 46°C, characterized by stable entry into the logarithmic phase and a prolonged stationary phase. It grew optimally at 2% ethanol and 4% NaCl, as well as at pH 3, and produced amylase, protease, and cellulase. When it was tested against six pathogenic fungi, it exhibited the highest inhibition rate against Eutypella microtheca (72.62%). Then, it was followed by 58.88% and 56.53% against Mortierella gemmifera and Talaromyces fuscoviridis, respectively; 39.57% against Mortierella elongata; 38.85% against Fusarium oxysporum; and 20.77% against Trichoderma harzianum. This research study provides a basis for greater biocontrol efficacy of B. velezensis in high-temperature environments and for developing microbial fungicides.

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