PJB-2025-330
In Vitro Propagation and acclimatization of Lavandula Angustifolia Mill.
Mehwish Yousaf
Abstract
Lavandula angustifolia Mill. belongs to Lamiaceae is an aromatic perennial and evergreen shrub woody in nature. It is used in food industries, perfumes, cosmetics and various medicines due to its essential oil. In order to increase its production with desired traits to a large scale it is necessary to establish protocol for techniques like micropropagation and acclimatization to fulfill its increasing demand in Pakistan. Its slow growth by conventional methods from seeds, limited propagation and excessive usage by different industries results in high production costs. The aim of this research work was to develop a protocol for multiplication through in vitro propagation and acclimatization of lavender. The nodal segments, apical meristem and lateral buds were pre surface sterilized with 50% ethanol for 30 seconds and 10% sodium hypochlorite for 10 minutes showed lowest contamination and high germination rate. MS media was used as nutrient medium for lavender growth. Different PGRs combinations of cytokinins and auxins were applied for micropropagation such as BAP, Kin, NAA, IBA etc. The best results for micropropagation was observed in MS media supplemented with 1mg/L Kin with 98% frequency and 2mg/L BAP with 97% frequency with highest number of shoot, root and leaves and highest shoot and root length in cm. For acclimatization, out of all potting mixtures Vermiculite + Perlite + Sand with 2:2:1 with 91.66% survival rate showed the most favourable outcomes for L. angustifolia. This method allows for rapid, conservational, disease free and cost-effective production of plants with desired traits through in vitro propagation, regardless of the season. The current investigation reports efficient protocol for rapid in vitro propagation under controlled suitable environment conditions in lab by providing unique genetic diversity ensuring a more sustainable future.
To Cite this article:
Under Review
We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More
