Paper Details

Abstract

Dillenia indica L. is a well-known medicinal plant with many pharmacological properties. Most of the biological activities are contributed by the wide variety of compounds present in D. indica. The objective of this study was to conserve the rare plant species of Dillenia indica and to investigate the effect of ZnO nanoparticles on their in vitro propagation. Various growth regulators, auxins, and cytokinins, were tested along with ZnO nanoparticles at different concentrations. The result showed that BAP at a 2 mg/L concentration had the most prominent effect among all the PGRs tested. Shoot induction (90.1%) was examined with the shoot initiation within 7.05±0.01f days of inoculation and gained a maximum length of 3.92±0.02a cm. The shoot number was found to be 8.22±0.02a after two subcultures. Apart from BAP, nanoparticles had a significantly positive effect on plant growth. ZnO nanoparticles enhanced the multiplication. The optimal concentration of ZnO nanoparticle was 250 mg/L, which increased the shoot induction (100%) with the shoot number 12.44±0.03a and shoot length 4.20±0.02acm after two subcultures. Nanoparticles not only enhanced shoot induction but also had a significant role in root induction. Rooting occurred only in the medium with an optimal concentration of nanoparticles in both microshoots and callus. For callus formation, the best media was found to be 2 mg/L BAP + 2 mg/L NAA. These findings demonstrate that nanoparticles are a useful tool in plant tissue culture and preservation initiatives because they not only encourage growth but also aid in reducing contamination-related in vitro losses in cultures.

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