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PJB-2025-297

NUTRITIONAL ANALYSIS, ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOSITION OF DIPLOTAXIS GRIFFITHII FROM SOON VALLEY, SALT RANGE, PAKISTAN

Amin Shah

Abstract

Diplotaxis griffithii is a medicinal plant in the Brassicaceae family. Historically, it has been used as a leafy vegetable. Recent studies show that this plant possesses high antioxidant, cytotoxic, and apoptotic activities, suggesting potential anticancer properties. The purpose of this study was to analyze the proximate composition, total phenolic compounds, and antioxidants of D. Griffithii. Extracts were prepared using ethanol and distilled water. For proximate analysis, shade-dried and sun-dried samples were examined. Results indicated that D. Griffithii contained the highest protein content in both samples, valued at 31.1633±0.0251% (shade-dried) and 31.4823±0.5540% (sun-dried). Analysis of ash, fiber, and moisture revealed good concentrations: sun-dried 24.0200±0.02%, 23.0133±0.01527%, and 8.2767±0.03055%; shade-dried 28.0133±0.01527%, 21.0233±0.02081%, and 10.9633±0.2193%. Fat content was low, 2.77±0.01% (shade-dried) and 1.25±0.02% (sun-dried).Total phenolic content was determined in aqueous and ethanol extracts, with the highest value 1568±1.00 mg/100g GAE in sun-dried aqueous extract. The highest flavonoid content was in shade-dried aqueous extract (229.630±3.70 mg/100g CE), while the least was in sun-dried ethanol extract (125.96±3.70 mg/100g CE). The highest antioxidant content was found in shade-dried ethanol extract (3641.08±2.70 µg/100g AAE) and the least in sun-dried aqueous extract (522.16±2.70 µg/100g AAE). This study reveals that D. Griffithii is a highly nutritious wild leafy vegetable with high antioxidant potential and phenolic composition.

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