PJB-2025-217
Salman Ahmed
Abstract
Introduction: Macrotyloma uniflorum (MU), Phaseolus lunatus (PL), and Phaseolus vulgaris (PV) beans are nutrient-rich legumes promoting digestion, heart health, and disease prevention. This study examined the inhibitory effects of aqueous seed infusions from MU, PL, and PV on calcium hydrogen phosphate dihydrate (Brushite) crystal growth, a precursor to kidney stones, contributing to high recurrence rates.
Material and methods: Using an in vitro gel technique, infusions (5–15%) and phytic acid (PA) as a standard were tested against Brushite. After 14 days, SEM, EDS, and FTIR analyses confirmed bioactive compound incorporation, disrupting crystal growth.
Results: MU showed the highest inhibition (62.79%), surpassing PA (55.81%), while PL had the lowest (33.72–40.69%). PV exhibited moderate inhibition (47.67–50%). SEM revealed crystal nuclei in 1PA, MU, and PV, with rough aggregates in PL and 15PV. EDS confirmed high inhibition in MU due to elemental disruptions. FTIR validated structural alterations, indicating MU’s strong potential as a natural preventive agent against Brushite stone formation.
Conclusion: Overall, different infusions exhibited significant inhibitory effects Brushite crystal formation while MU as most dominate, need further detail studies.
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