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Abstract

Tinospora crispa is a medicinal plant used in traditional medicine for its wide range of pharmacological activities. This study examined the phytochemical screening and qualitative data through gas chromatography-mass–mass spectrometry (GC–MS) analysis of nonpolar dichloromethane (DCM) extract of the aerial parts of T. Crispa. The screening confirmed the presence of steroids, terpenoids, fatty acids, tannins, saponins, coumarins, carbohydrates, resins, alkaloids, glycosides, cardiac glycosides, and proteins. However, anthocyanins, leucoanthocyanins, and emodins were not detected. GC–MS analysis identified a complex mixture of compounds, including oxygenated monoterpenes (e.g., 2-cyclohexen-1-one, 4-(3-hydroxybutyl)-3,5,5-trimethyl), long-chain ketones (e.g., 2-pentadecanone, 6,10,14-trimethyl), fatty acids (n-hexadecanoic and octadecanoic acids), and a variety of sterols and triterpenoids such as ethyl iso-allocholate, stigmasterol, α-amyrin, and betulin. Notably, a high relative abundance (43.85% area sum) of 6a,14a-methanopicene, perhydro-1,2,4a,6b,9,9,12a-heptamethyl-10-hydroxy was observed, suggesting that triterpenoids play a significant role in the plant's bioactivity. These findings provide a comprehensive chemical foundation for the traditional uses of T. crispa and indicate its potential as a source of novel therapeutic agents.

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