PJB-2025-188
Medium optimization of 1-deoxynojirimycin produced by Stenotrophomonas maltophilia-Y1 of mulberry endophytes
Jingjing Ye
Abstract
Stenotrophomonas maltophilia-Y1, a new endophytic strain was firstly isolated from mulberry leaves with strong production capacity of 1- deoxynojirimycin (DNJ) which indicated as an important potent α-glucosidase inhibitor. The medium to produce DNJ from S. maltophilia-Y1 using was optimized by the Plackett-Burman design (PBD) and response surface methodology (RSM). The PBD revealed that peptone and MgSO4 were identified as three significantly influencing DNJ production. RSM was applied to determine the mutual interactions between these three components and optimal levels for DNJ produce capacity. The optimized medium that the optimal media was composed of the following factors (g/L): maltose 40.0, peptone 14.285, urea 7.391, MgSO4 0.7706, and NaCl 1.0. This statistical optimization resulted in 2.27 times (428.800 mg/L) increase in DNJ production as compared to the initial yield of the basal medium.
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