Paper Details

PJB-2025-183

Antioxidant and antifungal effects of Nertera granadensis (Mutis ex L.F.) Druce and HPLC-MS determination of its phenolic compounds

Carlos Schneider, Carola Vergara, Claudia Moraga, Camila Zúñiga, Felipe Ruminot and Eduardo Navarrete
Abstract

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In this study the determination of biological effects of Nertera granadensis (Mutis ex L.f.) Druce, such as antioxidant and antifungal activities, was complemented with the qualitative analysis of phenolic compounds. It should be noted that the antioxidant and antifungal effects of N. granadensis have not been studied in previous studies. The antioxidant activities between four types of extracts obtained from the whole plant and the fruits were compared, and the antifungal effect of two types of extracts was assayed against the fungus Rhizoctonia solani. With the aim of evaluating the antioxidant effect, IC₅₀ values were calculated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and total phenolic content was determined by means of the Folin-Ciocalteu method. The antioxidant effect was also determined as gallic acid equivalents for the DPPH assay and as Trolox equivalents for the ABTS assay. Phenolic compounds contained in the whole plant and fruits of N. granadensis were analysed using high-performance liquid chromatography and mass spectrometry (HPLC-MS) analysis. The extract obtained with methanol from the leaves, stems, and roots of N. granadensis using Soxhlet extraction (WPM) showed a higher antioxidant effect according to lower IC₅₀ values of 2.8515 ± 0.0590 mg/mL and 0.7851 ± 0.0316 mg/mL, observed for the DPPH and ABTS assays, respectively. WPM also showed a fungistatic effect against R. solani. The results observed in this study revealed the antioxidant and antifungal effects of N. granadensis, and the HPLC-MS analysis allowed the identification of flavonols and hydroxycinnamic acid derivatives

To Cite this article: Schneider, C., C. Vergara, C. Moraga, C. Zúñiga, F. Ruminot and E. Navarrete. 2026. Antioxidant and antifungal effects of Nertera granadensis (Mutis ex L.F.) Druce and HPLC-MS determination of its phenolic compounds. Pak. J. Bot., 58(4): DOI: http://dx.doi.org/10.30848/PJB2026-4(4)  
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