Paper Details

PJB-2025-140

Micropropagation of blackberries in vitro on a chemically sterilized medium using sodium hypochlorite

Mikhail Sereda and Kasym Dzhebbarov
Abstract

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Blackberry (Rubus fruticosus L.) is a globally important berry crop. The process of micropropagation of plants allows for the production of large quantities of blackberry seedlings, but it is crucial to reduce costs. This research explores the possibility of reducing the cost of micropropagation by replacing the sterilization of the culture medium with chemical sterilization using sodium hypochlorite. In this study, we evaluated the effectiveness of micropropagation of the blackberry variety Brzezina on culture media that had been chemically sterilized with sodium hypochlorite. The microshoots of blackberry obtained from the In vitro collection were assessed at different stages of multiplication and rooting, using varying concentrations of active chlorine (0.001% to 0.008%). The study also included a thorough examination of the potential phytotoxicity of the culture media.. Throughout the research, the growth and development of plants In vitro were monitored, including the measurement of the effective photochemical quantum yield of photosystem II. The study determined the optimal concentrations of active chlorine (0.001% to 0.002%) that effectively sterilize the medium without negatively impacting the reproduction and rooting indices compared to the control. The fresh weight of new explants and the length of shoots produced during multiplication were significantly higher than in the case of the autoclaved medium. The blackberry seedlings grown on chemically sterilized medium successfully adapted to ex vitro conditions. Therefore, the method of chemically sterilizing nutrient media can be recommended for micropropagation of blackberries to reduce costs and increase productivity.

 

 

To Cite this article: Sereda, M. and K. Dzhebbarov. 2026. Micropropagation of blackberries in vitro on a chemically sterilized medium using sodium hypochlorite. Pak. J. Bot., 58(3): DOI: http://dx.doi.org/10.30848/PJB2026-3(16)  
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