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Abstract

The essential oils (EOs) extracted from Anvillea radiata Coss. & Dur. and Cotula cinerea Del. plants were studied for their: chemical composition, anti-inflammatory, antifungal and anti-gliotoxin activities, and synergistic interactions with each other and with other conventional anti-inflammatory and antifungal drugs. The chemical composition was analyzed by GC/MS and the anti-inflammatory activity was performed using Bovine Serum Protein Denaturation assay. The antifungal properties of the EOs were conducted using mycelial growth, determination of minimum inhibitory concentration (MIC), germination spores, mycelial biomass assay and anti-gliotoxin effect. The data showed that A. radiata was characterized by the abundance of (Z)-nerolidol (64.53%), Humulene epoxide II (10.59%) and epi-α-Cadinol (7.45%), while, C. cinerea was mainly composed of trans-thujone (17,32%), 1,8-Cineole (13.96%), cis-verbenyl acetate (12.61%), camphor (11.74%) and Santolina triene (10.80%). In regard to results of biological activities, the combination of the studied EOs between them and with synthetic drugs showed a significant decrease in their individual MICs of anti-inflammatory, inhibition of mycelial growth and germination spore activities against Aspergillus fumigatus and Aspergillus terreus cause aspergillosis. Similarly, the results revealed that there was a synergistic anti-gliotoxin effect when both EOs was combined with voriconazole, in contrast, an no interactive effect was observed when the EOs were combined with each other. The results obtained in this study suggest that A. radiata and C. cinerea EOs has considerable biological properties, while use in combinational, are recommended to enhance the anti-inflammatory and antifungal activities potency of their EOs for clinics and pharmaceutical applications. Key words: Essential oil, Biological activities, Aspergillosis, Drug, Synergy.

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