PJB-2025-31
Conserved genomic sequences are of evolutionary importance, as they give information regarding speciation and divergence of taxa. An important genomic sequence that has been used in the determination of plant taxonomic relationships is the gene for rbcL enzyme. With the increase in genomic data, the rcd1 that belongs to the sro (Similar to RCD-One) family of genes has been found conserved within all land plants. It bears a functional wwe domain encoding sequence which is easily amplified and exists in low copy number per genome. The nucleotides of this domain vary between different species of a plant family while it clusters plants belonging to a same family into statistically supported clades during phylogenetic analyses. This fact raised a need to identify the relationships it forms phylogenetically across larger variety of land plants. To achieve this objective, land plants belonging to diverse plant families were analysed using wwe and the obtained phylogenetic patterns were compared to those obtained using rbcL. For these analyses, eighteen sequences were amplified using rbcL barcode primers and others were retrieved from online databases. The trees were constructed using Physcomitrella patens as outgroup as it belongs to order Funariales which is quite divergent to the angiosperms. To the best of our knowledge, a phylogenetic analysis comparing rbcL gene with wwe domain of rcd1 has not been reported previously. The results demonstrate a potential for using the wwe domain as a molecular marker in DNA taxonomic studies, as it has shown to resolve all analysed plants at familial level with high bootstrap support.
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