PJB-2025-23
IN-SILICO APPROACH FOR PREDICTING HUMAN MICRORNA TARGETS AGAINST DENGUE VIRUS
Muhammad Ali Raza
Abstract
Small non-coding RNAs, or microRNAs, are crucial for cell proliferation, gene expression, and resistance to viral infection. This paper employed the computer-aided tools RNA22, RNAhybrid, and miRanda to predict miRNA-mRNA binding sites and identified potential miRNAs implicated in host response to dengue virus infection. The miRNA hsa-miR-2110 was discovered and its binding position on the dengue genome was further examined using this computational method. RNAhybrid, miRanda, and RNA22 suggested a number of binding sites within the protein-coding regions (NS1, NS2B, NS3, NS4A, NS4B, NS5, 2K, ancC, E, and prM). Additionally, target-site conservation study demonstrated that the core nucleotide sequence is necessary for potential miRNA binding across different virus strains, and comparison results indicated that the NS2A protein is the protein that human-derived miRNAs target the most frequently. These findings suggest that new treatment strategies for treating dengue virus infection may be able to target these miRNAs. Additionally, the molecular docking method was used to assess the binding capability of each miRNA and the NS2A protein as well as to predict their three-dimensional structures.
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Paper Repeat PJB-2025-20
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