PJB-2025-12
CALLUS INDUCTION IN MORINGA (MORINGAOLEIFERA LAM.)
Janani T
Abstract
MoringaoleiferaLam. is a highly significant tree due to its various parts possessing both nutritive and medicinal properties.Contemporary pharmaceuticals commonly rely on metabolites sourced from plants, with ongoing discoveries of novel products being made on a regular basis.However, the reliable and standardized provision of plant-based pharmaceuticals is often affected. In vitro plant tissue culture is a viable alternative for the synthesis of essential active compounds of plants, as it eliminates the need to rely on the plants and thus ensures independence from geographical conditions. The aim of this work is to produce an in vitro protocol for the cultivation of Moringaoleifera callus using leaf bit explants. The minimum duration for callus induction was determined to be seven days. The mean fresh weight of the callus was found to be 274.23a mg, while the mean dry weight was assessed to be 164.52a mg. Moringaoleifera Lam. callus cultures were successfully established from leaf bit explants using 2, 4-D concentration of 2.0 mg/l, which significantly enhanced callus formation.
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