Paper Details

Abstract

Ginger is an oriental spice which is commonly used as culinary and medicinal purposes. Conventionally, it is propagated by rhizomes which requires high seed rate for cultivation. Therefore, present study was designed to establish an in-vitro clonal propagation protocol for fast multiplication of ginger plants in short time. Sprouting buds were used as explants and data for days to shoot and root initiation, number and length of shoots and roots was recorded. Various growth regulators with different combinations and concentrations were tried to find the best combination. Benzylaminopurine (BAP) and kinetin were used for in-vitro shooting while Indole-3-acetic acid (IAA) for rooting purposes. Initially, these hormones were used in separate fashion to find the best level for shooting and rooting, then combined in single media to find the best combination. It was found that maximum number of shoots and roots were developed by using 4.0 mg/L of BAP, 0.5 mg/L of kinetin and 1.5 mg/L of IAA in same media. Plantlets were gradually exposed to natural environments and more than 80% survival rate was observed. Simultaneous development of in-vitro shoots and roots in same media was a major achievement of this research work. This optimized novel media not only reduce the time period for plant development but also a speedy process for in-vitro multiplication of ginger clones.

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