PJB-2024-606
PHYSIOLOGICAL RESPONSE OF RED PRICKLY ASH GERMPLASM RESOURCES TO LOW-TEMPERATURE STRESS AND COMPREHENSIVE EVALUATION OF COLD RESISTANCE
SHUQIN BAO
Abstract
To explore the physiological response of red prickly ash germplasms to low-temperature stress. Sixteen red prickly ash germplasms were used as experimental materials to study the differences in physiological indexes at 0, -10, -20, and -30 °C. The results showed that under low-temperature stress, the relative conductivity of the germplasms increased. The semi-lethal temperature of germplasms WCDHP, CCSJ, and HGHJ was lower than -10 °C. The semi-lethal temperatures of germplasms JYH, NLJ, and CJ were higher than -7 °C. The contents of soluble sugar and soluble protein first decreased, then increased, and then decreased with a decrease in temperature, while the content of free proline first increased and then decreased. The activity of superoxide dismutase first increased and then decreased, while the activities of peroxidase and catalase first increased, then decreased, and then increased. HGHJ, CCSJ, and SZT had the highest comprehensive scores of membership function, whereas CJ, JQWC, and NLJ exhibited the lowest comprehensive scores. Eight germplasms (including CCSJ, PTJ, and HGHJ) were screened by principal component analysis and showed strong cold resistance, whereas BYH, TSWC, and CJ exhibited weak cold resistance. In summary, CCSJ and HGHJ demonstrated strong cold resistance; NLJ and CJ demonstrated weak cold resistance.
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