PJB-2024-562
ANALYSIS OF GENETIC DIVERSITY FROM DIFFERENT GEOGRAPHICAL POPULATIONS IN BAUHINIA VARIEGATA LINN. WITH SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) MARKERS
JIUXIANG HUANG
Abstract
Bauhinia variegata Linn is a popular ornamental plant in South China and has medical, environmental remediation, and environmental biomonitoring values. The genetic diversity and differentiation among seven geographic populations of B. varigata that collected from different region in south China were analyzed using sequence-related amplified polymorphism (SRAP) markers. Using 13 primer pairs, 168 bands were generated. The percentage of polymorphic bands was 66.67%. The genetic Nei’s distance among different B. varigata populations ranged from 0.2548 to 0.0470. The general Nei's genetic diversity index was 0.2265. The general Shannon's information index was 0.3405. Genetic differentiation (Gst) among populations was 0.4580. The B. varigata population from Hong Kong was clustered into one separate group with other populations from mainland China. The SRAP data indicated that the B. varigata has certain genetic diversity and the gene flow occurred mainly intra-populations. The prepotency selection should be processed intra-population, and the germplasm introduction should focus on different regions for a wider collection range, which is in accordance with the expansive distribution of B. variegata.
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