PJB-2024-465
ANALYSIS OF OCIMUM BASCILLICUM L. EXTRACT FOR THE DEGRADATION OF OCHRATOXINS
Noor Fatima
Abstract
One of the primary challenges for global agricultural food production is ochratoxin exposure. Most essential crops, cereals, and dry fruits become impacted by ochratoxigenic fungi. This research's objective is to examine the antifungal effects of Ocimum bascillicum L. against Aspergillus niger van Tieghem. To do this, methanolic extract of O. bascillicum was made and tested in vitro against A. niger. Several conc. like, 1%, 2%, 3%, 4%, 5%, 6% and 7% of the test plant were prepared. The methanolic extract's 7% concentrations showed the strongest antifungal activity up to 93%. The O. basilicum antioxidant activity was evaluated using spectrophotometric 1, 1 diphenyl-2-picrylhydrazyl (DPPH) radical test. The results showed that when concentrations increased, the antioxidant potential increased as well. Methanolic extract of O. basilicum were separated into fractions of n-butanol, n-hexane, ethyl acetate and chloroform. Among them, the ethyl acetate fraction revealed strongest antifungal activity against O. basilicum. The potential antifungal compounds were identified by using GC-MS analysis of the ethyl acetate fraction. GC-MS analysis showed the presence of twenty five compounds including linalool, estragole, trans-alpha-bergamotene, bicyclodec-1-ene, 2-isopropyl-5-methyl-9-methylene, neophytadiene, n-hexadecanoic acid, phytol, oleic acid, 9,12 octadecadienoic acid, 9,12,15 octadecatrienoic acid, (z,z,z), octacosane, 1,19-eicosadiene, phytanic acid, heneicosane, 3-methyl, phenol, 2,2 methylenebis 1,1-dimethylethyl-4-methyl, tetracosane, glycerol palmitate, bis(2-ethylhexyl) phthalate, hexacosane, octadecane, squalene, pentadecanal, heptacosane, 2-dodecen-1-yl-succinic anhydride, 1-chloroeicosane. The plant extract was applied in vivo against ochratoxin producing fungus and the degradation of ochratoxin was assessed using HPLC. The ochratoxin degradation of O. basilicum by in vitro detoxification assay against pathogenic fungi (A. niger) was also performed. The TLC detection of ochratoxins from test fungi and plant sample result that O. basilicum effectively reduced the levels of ochratoxin in A. niger. Quantitative detection of ochratoxins by HPLC shows a great amount of reduction of OTA in experimental sample. The present study revealed that plant based extracts are efficient in detoxification of ochratoxins.
To Cite this article:
Not Recommended
We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More