PJB-2024-448
MORPHOLOGICAL AND MOLECULAR CONFIRMATION OF THE FIRST FINDING OF LECCINUM DURIUSCULUM (BOLETACEAE, BOLETALES) IN GLACIAL ROCKY MOUNTAINS OF HUNZA VALLEY (CENTRAL KARAKORUM), GILGIT, NORTHERN PAKISTAN
Samina Sarwar
Abstract
The of slate bolete Leccinum duriusculum is reported for the first time from Pakistan based on morphological, scanning electron microscopical, ecological and phylogenetic evidences. Sampling was done from glacial rocky mountains of Hunza valley (Central Karakorum), Gilgit, Northern Pakistan, dominated by Populus alba trees at their foothills. A complete macro- and micro-morphological description is provided and accompanied by colour images of fresh basidiomes in habitat and microscopic images with main anatomical features. Phylogenetic analysis by internal transcribed spacer (ITS) and larger subunit (LSU) sequences of nrDNA of the studied sample is also presented to substantiate the taxonomic identification of Leccinum duriusculum and ascertain its phylogenetic placement. Comparisons with closely allied, phenotypically and molecularly similar taxa are also given. To the best of our knowledge the present records represent the occurrence of this taxon in Pakistan.
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