Paper Details

Abstract

This study presents a streamlined protocol for the in vitro propagation of virus free banana plants using sucker as explants. The experiment focused on optimizing hormonal combinations for culture initiation, shoot multiplication, elongation, and root induction. Banana explants were cultured on MS medium supplemented with various concentrations of BAP, resulting in successful establishment of shoot initiation. Banana bunchy top disease, caused by Banana bunchy top virus (BBTV), is a serious disease that affects the productivity of banana to a greater extent. The production of virus-free planting material is an efficient method in controlling this disease and increase productivity. Followed to detect the presence of Banana Bunchy Top Virus (BBTV) in banana plants using DNA extraction and Polymerase Chain Reaction (PCR) techniques targeting the CP (Coat Protein) gene through primer amplification. The results of the tests showed no presence of the virus in the samples tested. This finding suggests the absence of BBTV in the examined banana plants, indicating a successful viral indexing procedure. And then Subsequent sub-culturing led to significant shoot proliferation, with a three to four-fold increase observed. The most productive hormonal combination for shoot multiplication involved MS supplemented with 5 mg/l BAP and 0.5 NAA. Elongated shoots were induced using IAA-supplemented MS medium, with the best results obtained at 6 mg/l IAA. Rooting was achieved within 35 days using a simplified protocol, contrasting with previous reports of longer durations. The established protocol offers a promising approach for large-scale in vitro disease free banana plantlet production, providing a straightforward and efficient method for commercial production.

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