PJB-2024-254
MULTIVARIATE ANALYSIS, MORPHOLOGICAL CHARACTERIZATION AND IN VITRO ORGANOGENESIS IN ELITE VARIETIES OF CARNATION (DIANTHUS CARYOPHYLLUS)
Sehrish Somaya
Abstract
Plant tissue culture techniques can be successfully adopted for rapid multiplication of the exclusive indigenous planting material. This study was conducted at the Plant Tissue Culture laboratory of Horticultural Research Institute for Floriculture and Landscaping, Islamabad, during the year 2018-2021. Three carnation varieties named Antigua, Montoyo and Tabasco were used as an explant material. Different combinations of growth regulators 6-Benzyleaminopurine (BAP) and Naphthaleneacetic Acid (NAA) were used to investigate the optimum concentration of both hormones for best shoot proliferation protocol. Each variety behaved differently producing variable results against the parameters tested. In case of Antigua, the combination of 4.0 mg/L BAP + 0.5 mg/L NAA proved to be the best by producing maximum number of shoots, shoot length and shoot proliferation percentage whereas, Montoyo and Tabasco showed satisfactory results on the medium supplemented with 3.0 mg/L BAP + 1.5 mg/L NAA and 4.0 mg/L BAP + 1.5 mg/L NAA respectively. After successful standardization of shoot proliferation protocol, the plantlets were transferred to the ½ strength MS medium enriched with 0.25 mg/l Indole-3-Butyric Acid (IBA) for root initiation. The rooted plantlets were transplanted in the small cells containing mixture of soil and leaf manure (1:1) for acclimatization.
Key Words: Carnation, BAP, NAA, Morphology and Multivariate analysis
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