PJB-2024-230
PLANT GROWTH PROMOTING ACTIVITY OF NATIVE BACILLUS SPECIES ON Zea mays L.
Semih Yılmaz
Abstract
Plant growth promoting bacteria (PGPR) are free-living microorganisms able to colonize on roots. There are mutual interactions between the bacteria and the plants. The objective of this study was to isolate Bacillus species from soil samples of Iraqi natural flora and screen them for the genes encoding growth related metabolites. The isolates bearing 1-aminocyclopropane-1-carboxylate deaminase, siderophore and acid phosphatase genes together were hypothesized to have high growth promotion and tested on maize under greenhouse conditions. Treatments included seed and seedling inoculation trials with three replications at concentrations of 108 CFU mL-1. The results were subjected to One-Way ANOVA and significant means were compared with Tukey’s test (p ≤ 0.05). Seed inoculation trials yielded better outcomes for shoot length (p = 0.000), shoot fresh weight (p = 0.000), shoot dry weight (p = 0.000), shoot diameter (p = 0.000), root volume (p = 0.000), root length (p = 0.041), root fresh weight (p = 0.000) and root dry weight (p = 0.005). Isolates SY29.1, KH28.1 and KH6.2 yielded significant increase in stem fresh weight. Promising isolates KH28.1 and KH18.2 resembled to Bacillus subtilis, KH16.2 to Bacillus sp., KH14.2 to Bacillus cereus and SY29.1 to Bacillus simplex upon analysis of 16S rDNA regions using NCBI’s Basic Local Alignment Search Tool. Seed inoculations yielded better outcomes than seedling inoculations for investigated parameters.
Keywords: Bacillus cereus, Bacillus simplex, Bacillus subtilis, Zea mays, ACC deaminase, Siderophore.
To Cite this article:
Not Recommended
We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More
