PJB-2024-201
Methodological and physiological study of seed vigor determination in Sinojackia xylocarpa Hu
Hao Cai
Abstract
Sinojackia xylocarpa Hu, a unique endangered plant endemic to China, requires seed banking as a crucial step for the preservation and expansion of its species diversity. The vigor of these seeds requires testing to ensure the collection of high-quality seeds. In this study, a method for the determination of seed vigor of Sinojackia xylocarpa Hu was selected by orthogonal experiment and response surface experiment. The results showed that the seeds were removed after 60 min treatment with H2SO4, then immersed in 0.91% tetrazolium solution, and stained in a constant incubation temperature at 39.72 ℃ in the dark for 8.93 h. Concurrently, a verification experiment on the same seed batch demonstrated a minimal difference (below 3%) in the embryos germination and staining rates of the seeds after H2SO4 treatment. In addition, the seeds before and after H2SO4 treatment were observed by scanning electron microscopy and paraffin sections. It was found that H2SO4 treatment not only had little damage to them, but also enhanced the penetration of tetrazolium solution and absorption of the water. As a result, this method presents an efficient and rapid means to evaluate the vigor of Sinojackia xylocarpa Hu seeds, thus significantly contributing to the conservation and utilization of Sinojackia xylocarpa Hu germplasm resources.
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