PJB-2024-196
Yufan Wang
Abstract
Peanut web blotch is one of the more prevalent and rapidly spreading diseases of peanut. In 1974, Marasas et al.identified the pathogen of peanut as Phoma arachidicola. In 2015, Chen et al.divided the pathogen of peanut web blotch into Didymella, so this paper used the latest name Didymella arachidicola. In this study, soil samples were collected from different crops and vegetable rhizosphere soils in different regions, and biocontrol fungus F11 was isolated and screened. The strain had a good inhibitory effect on Didymella arachidicola, and it was identified as Talaromyces sayulitensis by morphological identification and molecular biology. The fermentation medium and fermentation conditions were optimized by single factor test, orthogonal test and response surface test. The results showed that the optimum fermentation medium was corn gluten meal 24.33 g·L-1, diammonium hydrogenphosphate 2.56 g·L-1, and CaCO3 1.52 g·L-1. The model was established by Design Expert software, and the bacteriostatic rate under the above conditions was predicted to be 87.92%. The actual inhibition rate obtained after verification was 87.65%, which was close to the predicted value. Compared with 63.95% before optimization, it increased by 23.70%. The optimum fermentation conditions were as follows : liquid volume was 125 mL, temperature was 30°C, and fermentation time was 7 d.
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