PJB-2024-81
Systematic characterization, mineral analysis and valorization of ligno-cellulosic waste into sustainable indigenous Ganoderma lucidum production from Pakistan
Aneeqa Ghafoor
Abstract
Ganoderma lucidum is a remarkable medicinal macro fungus and widely known for its extra-ordinary healing qualities. Indigenous G. lucidum collected from Punjab, Pakistan was morpho-anatomically and phylogenetically characterized. After characterization, it was evaluated for vegetative and reproductive growth potential and also preceded for mineral analysis of both wild and cultivated basidiomata of G. lucidum. Amongst various tested nutrient agar media, maximum G. lucidum mycelial density and growth rate was noted on CEA medium at 32℃. For spawn production, barley grains found the best appropriate medium. Suitability of various types of lingo-cellulosic substrates was checked for better yield in minimum period of time. Combination of sawdust+wheat straw proved the efficient substrate for best yield with maximum no. of flushes. Small difference observed in essential and traces mineral quantities [except nickel] in both wild and cultivated basidiomata revealed the negligible effect of artificial substrates on mineral composition of G. lucidum. These findings concluded that indigenous G. lucidum have the capability for indoor cultivation on a number of locally available media and substrates. The cultivation of this tremendous medicinal mushroom on a large scale in Pakistan could prove very crucial in pharmaceutical perspective. Provision of cultures and fruiting bodies of G. lucidum to the food and therapeutic industries would eliminate their dependence on availability of G. lucidum for specific period of the year.
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