PJB-2024-78
DETECTION OF MYCOFLORA ASSOCIATED WITH SOYBEAN SEED AND ITS IMPACT ON SEED HEALTH
Twinkle
Abstract
An investigation was carried out to detect the mycoflora in soybean seeds. Seed samples were obtained through multiplication of different soybean varieties viz., Palam Soya, Shivalik and Hara Soya at the experimental farm of the Department of Plant Pathology, CSKHPKV Palampur. To determine the effect of chemical treatment on seed mycoflora, fungicide was applied to various soybean cultivars sown in the field. The presence of seed-borne fungi in soybean and their effect on seed health were evaluated using two methods viz., agar plate method and blotter paper method. A total of 13 fungal species were identified including Aspergillus niger, A. flavus, Rhizopus sp., Alternaria alternata, Penicillium sp., Colletotrichum truncatum, Trichoderma sp., Curvularia sp., Fusarium proliferatum, F. equiseti, Phoma sp., Cladosporium sp. and Pestalotiopsis sp. in different soybean seed samples obtained from treated and untreated plants. Among the two methods used for detection of mycoflora, the agar plate method resulted in higher frequency of fungal detection. Furthermore, the impact of these isolated fungi on seed germination, shoot length, root length, and seedling vigour index was assessed. It was observed that seeds inoculated with A. niger negatively affected all the parameters under study including seed germination, root length, shoot length and seedling vigour index (473).
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